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CoraLite® Plus 488-conjugated PDH E1 Alpha Monoclonal antibody

PDH E1 Alpha Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B3C10

Cat no : CL488-66119

Synonyms

PDHA, PDHA1, PDHCE1A, PDHE1 A type I, PHE1A



Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-66119 is PDH E1 Alpha Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PDH E1 Alpha fusion protein Ag12556
Full Name pyruvate dehydrogenase (lipoamide) alpha 1
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC002406
Gene Symbol PDHA1
Gene ID (NCBI) 5160
RRIDAB_2919287
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A.It is one of the 3 enzymes of the pyruvate dehydrogenase complex which is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA(PMID:7853374).It has 4 isoforms produced by alternative splicing.Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) and X-linked Leigh syndrome (X-LS).

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 PDH E1 Alpha antibody CL488-66119Download protocol
Standard Protocols
Click here to view our Standard Protocols