PDE6D Monoclonal antibody, PBS Only

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  WB analysis of Jurkat using 67565-1-Ig (same clone as 67565-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67565-1-PBS in a different storage buffer formulation.

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  WB analysis using 67565-1-Ig (same clone as 67565-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67565-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 67565-1-Ig (same clone as 67565-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67565-1-PBS in a different storage buffer formulation.

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  WB analysis of rat retina using 67565-1-Ig (same clone as 67565-1-PBS)

  rat retina tissue were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67565-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 67565-1-Ig (same clone as 67565-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67565-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67565-1-Ig (same clone as 67565-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67565-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67565-1-Ig (same clone as 67565-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67565-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67565-1-Ig (same clone as 67565-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67565-1-Ig (PDE6D antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67565-1-PBS in a different storage buffer formulation.

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  IHC staining of rat stomach using 67565-1-Ig (same clone as 67565-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 67565-1-Ig (PDE6D antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67565-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 67565-1-Ig (same clone as 67565-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67565-1-Ig (PDE6D antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67565-1-PBS in a different storage buffer formulation.

PDE6D Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67565-1-PBS

Clone No.1H4B6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Synonyms

PDED, 1H4B6

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Product Information

67565-1-PBS targets PDE6D in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PDE6D fusion protein Ag29563
• Full Name: phosphodiesterase 6D, cGMP-specific, rod, delta
• Calculated Molecular Weight: 17 KDa
• Observed Molecular Weight: 15-17 kDa
• GenBank Accession Number: NM_002601
• Gene Symbol: PDE6D
• Gene ID (NCBI): 5147
• RRID: AB_2882779
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O43924
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

PDE6D, also known as PDED, belongs to the PDE6D/unc-119 family. PDE6D is a ubiquitously expressed prenyl-binding protein. By binding to prenyl moieties, PDE6D mediates the shuttling of prenylated proteins between membranes. Thereby, it regulates the membrane association of Ras and Rap GTPases (PMID: 36672247). In addition, research has shown that PDE6D is overexpressed in liver cancer and correlated with tumor stages, grading, and ERK activation (PMID: 30901922). The calculated molecular weight of PDE6D is 17 kDa.