PDE6D Monoclonal antibody

PDE6D Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H4B6

Reviews (2)

Cat no : 67565-1-Ig

Synonyms

GMP PDE delta, PDE6D, PDED, Protein p17

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Validation Data Gallery

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  WB analysis of Jurkat using 67565-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67565-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67565-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67565-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 67565-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat retina using 67565-1-Ig

  rat retina tissue were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67565-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, HepG2 cells, human placenta tissue, rat retina tissue, mouse brain tissue, JAR cells, Pig brain tissue, Rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67565-1-Ig targets PDE6D in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, pig samples.

• Tested Reactivity: Human, Mouse, Rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PDE6D fusion protein Ag29563
• Full Name: phosphodiesterase 6D, cGMP-specific, rod, delta
• Calculated Molecular Weight: 17 KDa
• Observed Molecular Weight: 15-17 kDa
• GenBank Accession Number: NM_002601
• Gene Symbol: PDE6D
• Gene ID (NCBI): 5147
• RRID: AB_2882779
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDE6D, also known as PDED, belongs to the PDE6D/unc-119 family. PDE6D is a ubiquitously expressed prenyl-binding protein. By binding to prenyl moieties, PDE6D mediates the shuttling of prenylated proteins between membranes. Thereby, it regulates the membrane association of Ras and Rap GTPases (PMID: 36672247). In addition, research has shown that PDE6D is overexpressed in liver cancer and correlated with tumor stages, grading, and ERK activation (PMID: 30901922). The calculated molecular weight of PDE6D is 17 kDa.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Charlotte (Verified Customer) (07-24-2023)

We tested the antibody against PDE6 delta KO cells. We can see the antibody is very noisy but it is working.

• Applications: Western Blot
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: NIH-3T3

PDE6D Antibody Western Blot validation (1/2000 dilution) in NIH-3T3 (Cat no:67565-1-Ig)

FH

Criseyda (Verified Customer) (09-19-2022)

The antibody had background bands/nonspecific bands present but worked best in 5% Milk TBS-T (Blocking, Primary and Secondary) and not 3% BSA TBS-T (Blocking, Primary and Secondary).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: T47D

PDE6D Antibody Western Blot validation (1:1000 dilution) in T47D (Cat no:67565-1-Ig)