PDE10A Recombinant antibody, PBS Only (Capture/Detector)

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  WB analysis using 84869-1-RR (same clone as 84869-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84869-1-RR (PDE10A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84869-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01645-2

  Sandwich ELISA standard curve of MP01645-2, Human PDE10A Recombinant Matched Antibody Pair - PBS only. 84869-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag12771. 84869-1-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

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  Sample test of MP01645-2

  The mean PDE10A concentration was determined to be 62.34 ng/mL in HeLa cell extract based on a 1.00 mg/mL extract load, 60.49 ng/mL in Neuro-2a cell extract based on a 1.50 mg/mL extract load and 71.70 ng/mL in SH-SY5Y cell extract based on a 1.00 mg/mL extract load.

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  Cytometric bead array standard curve of MP01645-1

  Cytometric bead array standard curve of MP01645-1, PDE10A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84869-1-PBS. Detection antibody: 84869-3-PBS. Standard: Ag12771. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP01645-2

  Cytometric bead array standard curve of MP01645-2, PDE10A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84869-2-PBS. Detection antibody: 84869-1-PBS. Standard: Ag12771. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84869-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84869-1-RR against Human PDE10A were performed. The affinity constant is 3.37 nM.

PDE10A Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84869-1-PBS

Clone No.242357E12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

phosphodiesterase 10A, HSPDE10A, EC:3.1.4.17, cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A, 242357E12

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Product Information

84869-1-PBS targets PDE10A as part of a matched antibody pair:

MP01645-1: 84869-1-PBS capture and 84869-3-PBS detection (validated in Cytometric bead array)

MP01645-2: 84869-2-PBS capture and 84869-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PDE10A fusion protein Ag12771
• Full Name: phosphodiesterase 10A
• Calculated Molecular Weight: 779 aa, 88 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC104860
• Gene Symbol: PDE10A
• Gene ID (NCBI): 10846
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y233
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PDE10A (Phosphodiesterase 10A) is a single member of the PDE10 protein family, and it catalyzes both cAMP and cGMP hydrolysis (PMID: 34550322). The literature on PDE10A has been focused on psychiatric and neurological disorders because PDE10A expression is enriched in the striatal region of the brain under normal conditions (PMID: 12967715). Moreover, PDE10A expression is induced in various human tumor cell lines and PDE10A inhibition suppresses tumor cell growth (PMID: 37232184).