CoraLite® Plus 488-conjugated PDCD4 Monoclonal antibody

• Featured Product
• KD/KO Validated

PDCD4 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F1A11

Cat no : CL488-66100

Synonyms

H731, Nuclear antigen H731 like, PDCD4, Protein 197/15a

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Validation Data Gallery

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  WB analysis using CL488-66100

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66100 (PDCD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66100 targets PDCD4 in WB applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PDCD4 fusion protein Ag19239
• Full Name: programmed cell death 4 (neoplastic transformation inhibitor)
• Calculated Molecular Weight: 469 aa, 52 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC026104
• Gene Symbol: PDCD4
• Gene ID (NCBI): 27250
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A.