Tested Applications
Positive FC detected in | PHA treated human PBMCs |
Recommended dilution
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is N/A per 10^6 cells in a 100 µl suspension or N/A per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
PE-FcA98062 targets PD-L1/CD274 in applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Recombinant protein Predict reactive species |
Full Name | CD274 molecule |
Calculated Molecular Weight | 290 aa, 33 kDa |
GenBank Accession Number | BC074984 |
Gene Symbol | PD-L1 |
Gene ID (NCBI) | 29126 |
Conjugate | PE Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 496 nm, 565 nm / 578 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.
Protocols
Product Specific Protocols | |
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FC protocol for PE PD-L1/CD274 antibody PE-FcA98062 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |