FcZero-rAb™ PE Anti-Human PD-L1/CD274 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using PE-FcA98062

  1x10^6 PHA treated (right) or untreated (left) PBMCs were surface stained with APC Anti-Human CD3 and 5 ul FcZero-rAb™ PE Anti-Human PD-L1/CD274 Rabbit RecAb (PE-FcA98062, Clone: 240721H8). Cells were not fixed.

PD-L1/CD274 FcZero-rAb^TM Recombinant Antibody for FC

Cat No. PE-FcA98062

Clone No.240721H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD274, PD-L1, 240721H8, B7 H1, B7-H1

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Tested Applications

• Positive FC detected in: PHA treated human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-FcA98062 targets PD-L1/CD274 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PD-L1/CD274 fusion protein Eg1114
• Full Name: CD274 molecule
• Calculated Molecular Weight: 290 aa, 33 kDa
• GenBank Accession Number: BC074984
• Gene Symbol: PD-L1
• Gene ID (NCBI): 29126
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.