Anti-Mouse PD-L1/CD274 Rabbit Recombinant Antibody, PBS Only

PD-L1/CD274 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

240993E1

Cat no : 98166-1-PBS

Synonyms

Cd274, PD-L1, Pdcd1lg1, Pdcd1l1, PD L1

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Validation Data Gallery

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  FC experiment of mouse splenocytes using 98166-1-RR (same clone as 98166-1-PBS)

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L1/CD274 Rabbit Recombinant Antibody (98166-1-RR, Clone: 240993E1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD3. Cells were not fixed. This data was developed using the same antibody clone with 98166-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98166-1-RR (same clone as 98166-1-PBS)

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L1/B7-H1 Rabbit Recombinant Antibody (98166-1-RR, Clone:240993E1) (red) or 0.25 ug Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L1/B7-H1 Rabbit Recombinant Antibody (98166-1-RR, Clone: 240993E1) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98166-1-PBS in a different storage buffer formulation.

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Product Information

The immunogen of 98166-1-PBS is PD-L1/CD274 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD274 antigen
• Calculated Molecular Weight: 33 kDa
• GenBank Accession Number: NM_021893.3
• Gene Symbol: PD-L1
• Gene ID (NCBI): 60533
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.