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Anti-Mouse PD-L1/CD274 Rabbit Recombinant Antibody

PD-L1/CD274 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

240993E1

Cat no : 98166-1-RR

Synonyms

Cd274, PD-L1, Pdcd1lg1, Pdcd1l1, PD L1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse PD-L1/CD274 Rabbit Recombinant Antibody (98166-1-RR)

    FC experiment of mouse splenocytes using 98166-1-RR

    1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L1/CD274 Rabbit Recombinant Antibody (98166-1-RR, Clone: 240993E1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD3. Cells were not fixed.

  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse PD-L1/CD274 Rabbit Recombinant Antibody (98166-1-RR)

    FC experiment of mouse splenocytes using 98166-1-RR

    1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L1/B7-H1 Rabbit Recombinant Antibody (98166-1-RR, Clone:240993E1) (red) or 0.25 ug Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L1/B7-H1 Rabbit Recombinant Antibody (98166-1-RR, Clone: 240993E1) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

Tested Applications

Positive FC detected inanti-CD3/CD28 treated mouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98166-1-RR is PD-L1/CD274 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name CD274 antigen
Calculated Molecular Weight33 kDa
GenBank Accession NumberNM_021893.3
Gene Symbol PD-L1
Gene ID (NCBI) 60533
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.

Protocols

Product Specific Protocols
FC protocol for PD-L1/CD274 antibody 98166-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols