PD-L1/CD274 Recombinant antibody, PBS Only (Capture/Detector)

PD-L1/CD274 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240721D2

Cat no : 83600-6-PBS

Synonyms

CD274, PD-L1, PD L1, hPD-L1, B7-H1

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00601-4

  Sandwich ELISA standard curve of MP00601-4, Human PD-L1/CD274 Recombinant Matched Antibody Pair - PBS only. 83600-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1114. 83600-6-PBS was HRP conjugated as the detection antibody. Range: 3.9-250 pg/mL.

• 

  Sample test of MP00601-4

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days or 5 days. For the PBMC supernatant cultured for 3 days, the mean PD-L1/CD274 concentration was determined to be 7.7 pg/mL in unstimulated PBMC supernatant, 108.4 pg/mL in PHA stimulated PBMC supernatant. For the PBMC supernatant cultured for 5 days, the mean PD-L1/CD274 concentration was determined to be 11.0 pg/mL in unstimulated PBMC supernatant, 160.7 pg/mL in PHA stimulated PBMC supernatant.

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  Sample test of MP00601-4

  Urine of seven individual healthy human donors was measured. The PD-L1/CD274 concentration of detected samples was determined to be 10.8 pg/mL with a range of 0.7 - 33.1 pg/mL.

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  Sample test of MP00601-4

  The mean PD-L1/CD274 concentration was determined to be 36,368.3 pg/mL in MDA-MB-231 cell extract based on a 2.3 mg/mL extract load.

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  Sample test of MP00601-4

  Serum of sixteen individual healthy human donors was measured. The PD-L1/CD274 concentration of detected samples was determined to be 75.1 pg/mL with a range of 34.4-168.1 pg/mL.

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  Cytometric bead array standard curve of MP00601-3

  Cytometric bead array standard curve of MP00601-3, PD-L1/CD274 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83600-6-PBS. Detection antibody: 83600-4-PBS. Standard: Eg1114. Range: 0.156-20 ng/mL.

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Product Information

83600-6-PBS targets PD-L1/CD274 as part of a matched antibody pair:

MP00601-3: 83600-6-PBS capture and 83600-4-PBS detection (validated in Cytometric bead array)

MP00601-4: 83600-7-PBS capture and 83600-6-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD274 molecule
• Calculated Molecular Weight: 290 aa, 33 kDa
• GenBank Accession Number: BC074984
• Gene Symbol: PD-L1
• Gene ID (NCBI): 29126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.