FITC Plus Anti-Human PD-L1 (B7-H1) (29E.2A3)

PD-L1 (B7-H1) Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

Human

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

29E.2A3

Cat no : FITC-65081

Synonyms

B7 H, B7 H1, B7 homolog 1, B7H1, CD274, CD274 molecule, PD L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1, PD-L1 (B7-H1), Programmed death ligand 1

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Validation Data Gallery

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  FC experiment of human PBMCs using FITC-65081

  1x10^6 PHA treated (3 days) human PBMCs were surface stained with 5 ul FITC Plus Anti-Human PD-L1 (B7-H1) (FITC-65081, Clone:29E.2A3) (red) or FITC Plus Mouse IgG2b Isotype Control (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul FITC Plus Anti-Human PD-L1 (B7-H1) (FITC-65081, Clone:29E.2A3) (black dashed). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65081

  1x10^6 PHA treated (3 days) human PBMCs were surface stained with CL750 Anti-Human CD3 and 5 ul FITC Plus Anti-Human PD-L1 (B7-H1) (FITC-65081, Clone:29E.2A3) (right) or FITC Plus Mouse IgG2b Isotype Control (left). 1x10^6 untreated human PBMCs were surface stained with 5 ul FITC Plus Anti-Human PD-L1 (B7-H1) (FITC-65081, Clone:29E.2A3) (center). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: PHA treated human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65081 targets PD-L1 (B7-H1) in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Full length human PD-L1
• Full Name: CD274 molecule
• GenBank Accession Number: BC069381
• Gene Symbol: CD274
• Gene ID (NCBI): 29126
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.