Mouse PD-L1/B7-H1 Recombinant antibody, PBS Only

PD-L1/B7-H1 Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240993C9

Cat no : 83906-6-PBS

Synonyms

Cd274, PD-L1, Pdcd1lg1, Pdcd1l1, PD L1

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Validation Data Gallery

• 

  WB analysis of mouse heart using 83906-6-RR (same clone as 83906-6-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 83906-6-RR (PD-L1/B7-H1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83906-6-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 83906-6-RR (same clone as 83906-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83906-6-RR (PD-L1/B7-H1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83906-6-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 83906-6-RR (same clone as 83906-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83906-6-RR (PD-L1/B7-H1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83906-6-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83906-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83906-6-RR against Mouse PD-L1/B7-H1 were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 83906-6-PBS is PD-L1/B7-H1 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD274 antigen
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: NM_021893.3
• Gene Symbol: PD-L1
• Gene ID (NCBI): 60533
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.