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PD-L1/B7-H1 Recombinant antibody

PD-L1/B7-H1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240993C9

Cat no : 83906-6-RR

Synonyms

Cd274, PD-L1, Pdcd1lg1, Pdcd1l1, PD L1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using PD-L1/B7-H1 Recombinant antibody (83906-6-RR)

    WB analysis of mouse heart using 83906-6-RR

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 83906-6-RR (PD-L1/B7-H1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using PD-L1/B7-H1 Recombinant antibody (83906-6-RR)

    IHC staining of mouse spleen using 83906-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83906-6-RR (PD-L1/B7-H1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using PD-L1/B7-H1 Recombinant antibody (83906-6-RR)

    IHC staining of mouse spleen using 83906-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83906-6-RR (PD-L1/B7-H1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83906-6-RR

    Affinity and Kinetic Characterization of 83906-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83906-6-RR against Mouse PD-L1/B7-H1 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inmouse heart tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83906-6-RR is PD-L1/B7-H1 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name CD274 antigen
Calculated Molecular Weight33 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberNM_021893.3
Gene Symbol PD-L1
Gene ID (NCBI) 60533
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.

Protocols

Product Specific Protocols
WB protocol for PD-L1/B7-H1 antibody 83906-6-RRDownload protocol
IHC protocol for PD-L1/B7-H1 antibody 83906-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols