APC Anti-Human PD-L1/CD274 (29E.2A3)

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  FC experiment of human peripheral blood lymphocytes using APC-65081

  1X10^6 PHA-stimulated (5 ug/ml, 3 days) human peripheral blood lymphocytes were surface stained with 5 ul APC Anti-Human PD-L1 (B7-H1) (APC-65081, Clone: 29E.2A3) (blue) or isotype control antibody (black). Cells were not fixed.

PD-L1/CD274 Monoclonal Antibody for FC

Cat No. APC-65081

Clone No.29E.2A3

Publications(11)

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

human

Applications

IHC, IF, FC

Synonyms

CD274, PD-L1, 29E.2A3, B7 H1, B7-H1

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Tested Applications

• Positive FC detected in: human peripheral blood lymphocytes

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 1 publications below
• FC: See 8 publications below

Product Information

APC-65081 targets PD-L1/CD274 in IHC, IF, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Full length human PD-L1

• Full Name: CD274 molecule
• GenBank Accession Number: BC069381
• Gene Symbol: PD-L1
• Gene ID (NCBI): 29126
• RRID: AB_2882979
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: Q9NZQ7
• Storage Buffer: PBS with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	FC	Adv Sci (Weinh); Inhibition of CK2/ING4 Pathway Facilitates Non-Small Cell Lung Cancer Immunotherapy; Authors - Qian Gou
		J Immunother Cancer; eEF2K promotes PD-L1 stabilization through inactivating GSK3β in melanoma.; Authors - Xisha Chen
human	FC	Oncogene; Dual role of pseudogene TMEM198B in promoting lipid metabolism and immune escape of glioma cells; Authors - Ying Zhan
human	FC	iScience; Cancer-associated fibroblasts promote enzalutamide resistance and PD-L1 expression in prostate cancer through CCL5-CCR5 paracrine axis; Authors - Zhi Xiong
		Cancer Sci; PPARγ inhibited tumor immune escape by inducing PD-L1 autophagic degradation; Authors - Qian Gou
human	FC	Stem Cell Res Ther; Glioma-associated mesenchymal stem cells-mediated PD-L1 expression is attenuated by Ad5-Ki67/IL-15 in GBM treatment.; Authors - Qing Zhang