PCYT1A Recombinant antibody, PBS Only

PCYT1A Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230393B4

Cat no : 83100-1-PBS

Synonyms

PCYT1, CTPCT, CT A, CCT alpha, CCT A

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Validation Data Gallery

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  WB analysis using 83100-1-RR (same clone as 83100-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83100-1-RR (PCYT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83100-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 83100-1-RR (same clone as 83100-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83100-1-RR (PCYT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83100-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 83100-1-RR (same clone as 83100-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PCYT1A antibody (83100-1-RR, Clone: 230393B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83100-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 83100-1-RR (same clone as 83100-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human PCYT1A (83100-1-RR, Clone:230393B4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83100-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83100-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83100-1-RR against Human PCYT1A were performed. The affinity constant is below 1 pM.

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Product Information

83100-1-PBS targets PCYT1A in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PCYT1A fusion protein Ag34268
• Full Name: phosphate cytidylyltransferase 1, choline, alpha
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 38-41 kDa
• GenBank Accession Number: NM_005017
• Gene Symbol: PCYT1A
• Gene ID (NCBI): 5130
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.