PCNA Recombinant antibody, PBS Only

PCNA Uni-rAb^TM Recombinant Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240306B9

Cat no : 81302-6-PBS

Synonyms

proliferating cell nuclear antigen, Cyclin, 240306B9

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Validation Data Gallery

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  WB analysis using 81302-6-RR (same clone as 81302-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81302-6-RR (PCNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81302-6-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 81302-6-RR (same clone as 81302-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81302-6-RR (PCNA antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81302-6-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 81302-6-RR (same clone as 81302-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81302-6-RR (PCNA antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81302-6-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 81302-6-RR (same clone as 81302-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using PCNA antibody (81302-6-RR, Clone: 240306B9 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81302-6-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 81302-6-RR (same clone as 81302-6-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug PCNA Recombinant antibody (81302-6-RR, Clone:240306B9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 81302-6-PBS in a different storage buffer formulation.

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Product Information

The immunogen of 81302-6-PBS is PCNA Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PCNA fusion protein Ag0277
• Full Name: proliferating cell nuclear antigen
• Calculated Molecular Weight: 29 kDa/31 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC000491
• Gene Symbol: PCNA
• Gene ID (NCBI): 5111
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).