HRP-conjugated PCMT1 Monoclonal antibody

PCMT1 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB

Conjugate

HRP

CloneNo.

3C8G8

Cat no : HRP-60172

Synonyms

PCMT1

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Validation Data Gallery

• 

  WB analysis of HEK-293 using HRP-60172

  HEK-293 cells were subjected to SDS PAGE followed by western blot with HRP-60172 (PCMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

HRP-60172 targets PCMT1 in WB applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PCMT1 fusion protein Ag6277
• Full Name: protein-L-isoaspartate (D-aspartate) O-methyltransferase
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25-27 kDa
• GenBank Accession Number: BC008748
• Gene Symbol: PCMT1
• Gene ID (NCBI): 5110
• RRID: AB_2920511
• Conjugate: HRP
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

The PCMT1 gene encodes the protein repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase, which is known to protect certain neural cells against Bax-induced apoptosis.