PCM1 Recombinant antibody, PBS Only

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  WB analysis using 84979-1-RR (same clone as 84979-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84979-1-RR (PCM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84979-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 84979-1-RR (same clone as 84979-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84979-1-RR (PCM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84979-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84979-1-RR (same clone as 84979-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84979-1-RR (PCM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84979-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84979-1-RR (same clone as 84979-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84979-1-RR (PCM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84979-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84979-1-RR (same clone as 84979-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PCM1 antibody (84979-1-RR, Clone: 242199A9 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84979-1-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 84979-1-RR (same clone as 84979-1-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug PCM1 Recombinant antibody (84979-1-RR, Clone:242199A9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84979-1-PBS in a different storage buffer formulation.

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  FC experiment of K562 using 84979-1-RR (same clone as 84979-1-PBS)

  1x10^6 K562 were intracellularly stained with 0.25 ug PCM1 Recombinant antibody (84979-1-RR, Clone:242199A9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84979-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84979-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84979-1-RR against Human PCM1 were performed. The affinity constant is 21.0 nM.

PCM1 {ptg:Brand} Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 84979-1-PBS

Clone No.242199A9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

pericentriolar material 1, PCM-1, PCM 1, hPCM-1, hPCM 1

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Product Information

84979-1-PBS targets PCM1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PCM1 fusion protein Ag13977
• Full Name: pericentriolar material 1
• Calculated Molecular Weight: 2024 aa, 228 kDa
• Observed Molecular Weight: 245-320 kDa
• GenBank Accession Number: BC000453
• Gene Symbol: PCM1
• Gene ID (NCBI): 5108
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q15154
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Pericentriolar material 1 (PCM1) is a component of centriolar satellites, which are electron dense granules scattered around centrosomes (PMID: 10579718). PCM1 is required for centrosome assembly and function. It acts as a scaffold to target several proteins to the centrosome in a dynein motor-dependent manner and regulate microtubular dynamics (PMID:18762586; 12403812). It is also involved in the biogenesis of cilia (PMID: 18772192). Chromosomal aberrations involving PCM1 gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. (PMID: 10980597; 16091753; 15805263; 16424865).