CoraLite® Plus 488-conjugated PCK1 Monoclonal antibody
PCK1 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
mouse, rat, pig, human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2F11B5
Cat no : CL488-66862
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-66862 targets PCK1 in IF/ICC applications and shows reactivity with mouse, rat, pig, human samples.
Tested Reactivity | mouse, rat, pig, human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | PCK1 fusion protein Ag9720 |
Full Name | phosphoenolpyruvate carboxykinase 1 (soluble) |
Calculated Molecular Weight | 622 aa, 69 kDa |
Observed Molecular Weight | 65-69 kDa |
GenBank Accession Number | BC023978 |
Gene Symbol | PCK1 |
Gene ID (NCBI) | 5105 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 PCK1 antibody CL488-66862 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |