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  • KD/KO Validated

PCGF6 Polyclonal antibody

PCGF6 Polyclonal Antibody for WB, IHC, ELISA, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, CoIP, ChIP, ELISA, FC (Intra)

Conjugate

Unconjugated

Cat no : 24103-1-AP

Synonyms

MBLR, PCGF6, polycomb group ring finger 6, RING finger protein 134, RNF134



Tested Applications

Positive WB detected inHEK-293 cells, HEK-293T cells, PC-13 cells, mouse liver tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4800
Immunohistochemistry (IHC)IHC : 1:150-1:600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24103-1-AP targets PCGF6 in WB, IP, IHC, CoIP, ChIP, ELISA, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCGF6 fusion protein Ag21124
Full Name polycomb group ring finger 6
Calculated Molecular Weight 352 aa, 39 kDa
Observed Molecular Weight40-50 kDa
GenBank Accession NumberBC010235
Gene Symbol PCGF6
Gene ID (NCBI) 84108
RRIDAB_2737369
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCGF6, also named as MBLR or RNF134, is a 350 amino acid protein, which contains one RING-type zinc finger. PCGF6 localizes in the nucleus and is widely expressed in many tissues. PCGF6 as a transcriptional repressor may modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase.

Protocols

Product Specific Protocols
WB protocol for PCGF6 antibody 24103-1-APDownload protocol
IHC protocol for PCGF6 antibody 24103-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell Stem Cell

SUMO Safeguards Somatic and Pluripotent Cell Identities by Enforcing Distinct Chromatin States.

Authors - Jack-Christophe Cossec
mouse

Sci Adv

The SAM domain-containing protein 1 (SAMD1) acts as a repressive chromatin regulator at unmethylated CpG islands.

Authors - Bastian Stielow
mouseWB

Nat Commun

E2F6 initiates stable epigenetic silencing of germline genes during embryonic development.

Authors - Thomas Dahlet
mouseWB

Nat Commun

Repression of germline genes by PRC1.6 and SETDB1 in the early embryo precedes DNA methylation-mediated silencing.

Authors - Kentaro Mochizuki
  • KO Validated
humanWB,CoIP

Nat Commun

PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression.

Authors - Xianchun Lan
  • KD Validated
humanWB

Elife

Loss of MGA repression mediated by an atypical polycomb complex promotes tumor progression and invasiveness.

Authors - Haritha Mathsyaraja

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Aktan (Verified Customer) (12-24-2019)

The blot is subcellular fractionation of the cell nuclei using increasing concentrations of salt. Last two lanes are chromatin fractions. After electrophoresis and transfer, 5%BSA in PBST was used as blocker for 1h. The primary antibody is diluted in blocker 1:500 and the blot is incubated o/n at cold. The bands are detected using licor secondary antibodies and Licor imager.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: E14Tg mouse embryonic stem cells
PCGF6 Antibody Western Blot, validation (1:500 dilution) in E14Tg mouse embryonic stem cells (Cat no:24103-1-AP)