|Positive WB detected in||HEK-293 cells, PC-13 cells, HEK-293T cells, mouse liver tissue|
|Positive IHC detected in||mouse testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4800|
|Immunohistochemistry (IHC)||IHC : 1:150-1:600|
|Sample-dependent, check data in validation data gallery|
24103-1-AP targets PCGF6 in WB, IP, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PCGF6 fusion protein Ag21124|
|Full Name||polycomb group ring finger 6|
|Calculated molecular weight||352 aa, 39 kDa|
|Observed molecular weight||40-50 kDa|
|GenBank accession number||BC010235|
|Gene ID (NCBI)||84108|
|Purification Method||Antigen Affinity purified|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
PCGF6, also named as MBLR or RNF134, is a 350 amino acid protein, which contains one RING-type zinc finger. PCGF6 localizes in the nucleus and is widely expressed in many tissues. PCGF6 as a transcriptional repressor may modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase.
Cell Stem Cell
SUMO Safeguards Somatic and Pluripotent Cell Identities by Enforcing Distinct Chromatin States.
The SAM domain-containing protein 1 (SAMD1) acts as a repressive chromatin regulator at unmethylated CpG islands.
E2F6 initiates stable epigenetic silencing of germline genes during embryonic development.
Repression of germline genes by PRC1.6 and SETDB1 in the early embryo precedes DNA methylation-mediated silencing.
PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression.
Loss of MGA repression mediated by an atypical polycomb complex promotes tumor progression and invasiveness.
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Aktan (Verified Customer) (12-24-2019)
The blot is subcellular fractionation of the cell nuclei using increasing concentrations of salt. Last two lanes are chromatin fractions. After electrophoresis and transfer, 5%BSA in PBST was used as blocker for 1h. The primary antibody is diluted in blocker 1:500 and the blot is incubated o/n at cold. The bands are detected using licor secondary antibodies and Licor imager.