PCCB Monoclonal antibody, PBS Only (Capture/Detector)

• Featured Product
• KD/KO Validated

PCCB Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A9A5

Cat no : 66501-1-PBS

Synonyms

Propionyl-CoA carboxylase beta chain, mitochondrial, Propanoyl-CoA:carbon dioxide ligase subunit beta, PCCase subunit beta, EC:6.4.1.3, 1A9A5

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Validation Data Gallery

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  WB analysis of HepG2 using 66501-1-Ig (same clone as 66501-1-PBS)

  WB result of PCCB antibody (66501-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PCCB transfected HepG2 cells. This data was developed using the same antibody clone with 66501-1-PBS in a different storage buffer formulation.

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  WB analysis using 66501-1-Ig (same clone as 66501-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66501-1-Ig (PCCB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66501-1-PBS in a different storage buffer formulation.

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  WB analysis using 66501-1-Ig (same clone as 66501-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66501-1-Ig (PCCB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66501-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66501-1-Ig (same clone as 66501-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66501-1-Ig (PCCB antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66501-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66501-1-Ig (same clone as 66501-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66501-1-Ig (PCCB antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66501-1-PBS in a different storage buffer formulation.

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  IF Staining of human kidney using 66501-1-Ig (same clone as 66501-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using 66501-1-Ig (PCCB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66501-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50335-2

  Cytometric bead array standard curve of MP50335-2, PCCB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66501-2-PBS. Detection antibody: 66501-1-PBS. Standard:Ag1608. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP50335-3

  Cytometric bead array standard curve of MP50335-3, PCCB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66501-1-PBS. Detection antibody: 66501-3-PBS. Standard:Ag1608. Range: 0.781-100 ng/mL.

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Product Information

66501-1-PBS targets PCCB as part of a matched antibody pair:

MP50335-2: 66501-2-PBS capture and 66501-1-PBS detection (validated in Cytometric bead array)

MP50335-3: 66501-1-PBS capture and 66501-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PCCB fusion protein Ag1608
• Full Name: propionyl Coenzyme A carboxylase, beta polypeptide
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC013768
• Gene Symbol: PCCB
• Gene ID (NCBI): 5096
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.