PBLD Monoclonal antibody, PBS Only

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  WB analysis of pig liver using 68317-1-Ig (same clone as 68317-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68317-1-Ig (PBLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68317-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig kidney using 68317-1-Ig (same clone as 68317-1-PBS)

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 68317-1-Ig (PBLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68317-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 68317-1-Ig (same clone as 68317-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PBLD antibody (68317-1-Ig, Clone: 1G11D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68317-1-PBS in a different storage buffer formulation.

PBLD Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 68317-1-PBS

Clone No.1G11D4

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

FLJ14767, FLJ35507, MAWBP, MAWD binding protein, MAWDBP, PBLD

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Product Information

68317-1-PBS targets PBLD in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, pig samples.

• Tested Reactivity: Human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PBLD fusion protein Ag23827
• Full Name: phenazine biosynthesis-like protein domain containing
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC009738
• Gene Symbol: PBLD
• Gene ID (NCBI): 64081
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P30039
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PBLD, also termed as MAWBP, was first identified from a human liver cDNA library using a yeast two-hybrid technique by Iriyama et al. As the only representative of phenazine biosynthesis-like protein family, PBLD is widely expressed in human tissues, such as brain, heart, lung, liver, pancreas, kidney, and placenta. Its expression is elevated in several diseases processes, including insulin resistance, folate deficiency, and hypotension. PBLD has been proved to be involved in gastric carcinogenesis, PBLD expression was attenuated in gastric cancer (GC) tissues, and negatively regulated the growth and invasion in gastric cancer cell-lines by inhibiting TGF-β1-induced EMT (Epithelial-Mesenchymal Transition).(PMID: 11355021, 17929853, 23687415, 26594798)