PARP1 Recombinant antibody, PBS Only

PARP1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse, human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3N19

Cat no : 80174-1-PBS

Synonyms

ARTD1, ADPRT1, ADPRT 1, ADPRT, ADP-ribosyltransferase diphtheria toxin-like 1

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Validation Data Gallery

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  WB analysis using 80174-1-RR (same clone as 80174-1-PBS)

  Jurkat cells treated with Staurosporin were subjected to SDS PAGE followed by western blot with 80174-1-RR (PARP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

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  WB analysis using 80174-1-RR (same clone as 80174-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80174-1-RR (PARP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80174-1-RR (same clone as 80174-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80174-1-RR (PARP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 80174-1-RR (same clone as 80174-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80174-1-RR (PARP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 80174-1-RR (same clone as 80174-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80174-1-RR (PARP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 80174-1-RR (same clone as 80174-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80174-1-RR (PARP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 80174-1-RR (same clone as 80174-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PARP1 antibody (80174-1-RR, Clone: 3N19 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 80174-1-RR (same clone as 80174-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PARP1 antibody (80174-1-RR, Clone: 3N19 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 80174-1-RR (same clone as 80174-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using PARP1 antibody (80174-1-RR, Clone: 3N19 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 80174-1-RR (same clone as 80174-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PARP1 antibody (80174-1-RR, Clone: 3N19 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80174-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80174-1-RR against Human PARP1 were performed. The affinity constant is 0.639 nM.

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Product Information

80174-1-PBS targets PARP1 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

• Tested Reactivity: rat, mouse, human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PARP1 fusion protein Ag4193
• Full Name: poly (ADP-ribose) polymerase 1
• Calculated Molecular Weight: 1014 aa, 113 kDa
• Observed Molecular Weight: 113-116, 89 kDa
• GenBank Accession Number: BC037545
• Gene Symbol: PARP1
• Gene ID (NCBI): 142
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kDa. This antibody was generated against the C-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.