PARL Recombinant antibody, PBS Only

• 

  WB analysis of MCF-7 using 85167-1-RR (same clone as 85167-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 85167-1-RR (PARL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85167-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85167-1-RR (same clone as 85167-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85167-1-RR (PARL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85167-1-PBS in a different storage buffer formulation.

PARL {ptg:Brand} Recombinant Antibody for WB, Indirect ELISA

Cat No. 85167-1-PBS

Clone No.242171C12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

PRO2207, Presenilin-associated rhomboid-like protein, mitochondrial, P-beta, Pbeta, EC:3.4.21.105

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Product Information

85167-1-PBS targets PARL in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PARL fusion protein Ag24789
• Full Name: presenilin associated, rhomboid-like
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC014058
• Gene Symbol: PARL
• Gene ID (NCBI): 55486
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9H300
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

PARL (Presenilin associated rhomboid like), is a member of the rhomboid family of intramembrane serine proteases that is localized to the inner mitochondrial membrane. PARL has 2 isoforms produced by alternative splicing with the molecular mass of 42 kDa and 37 kDa. PARL regulates mitochondrial remodeling and apoptosis through regulated substrate proteolysis. Proteolytic processing of PARL may result in the release of a small peptide, P-beta, which may transit to the nucleus. The self-regulated cleavage of PARL at positions 52-53 (α-site) and 77--78 (β-site) produce two cleaved forms of PARL named MAMP and PACT with the molecular mass of 36 kDa and 33 kDa respectively (PMID: 14732705, 28178523).