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PARL Recombinant antibody

PARL Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242171C12

Cat no : 85167-1-RR

Synonyms

PRO2207, Presenilin-associated rhomboid-like protein, mitochondrial, P-beta, Pbeta, EC:3.4.21.105

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PARL Recombinant antibody (85167-1-RR)

    WB analysis of MCF-7 using 85167-1-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 85167-1-RR (PARL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PARL Recombinant antibody (85167-1-RR)

    WB analysis using 85167-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85167-1-RR (PARL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85167-1-RR targets PARL in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PARL fusion protein Ag24789
Full Name presenilin associated, rhomboid-like
Calculated Molecular Weight 42 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC014058
Gene Symbol PARL
Gene ID (NCBI) 55486
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PARL (Presenilin associated rhomboid like), is a member of the rhomboid family of intramembrane serine proteases that is localized to the inner mitochondrial membrane. PARL has 2 isoforms produced by alternative splicing with the molecular mass of 42 kDa and 37 kDa. PARL regulates mitochondrial remodeling and apoptosis through regulated substrate proteolysis. Proteolytic processing of PARL may result in the release of a small peptide, P-beta, which may transit to the nucleus. The self-regulated cleavage of PARL at positions 52-53 (α-site) and 77--78 (β-site) produce two cleaved forms of PARL named MAMP and PACT with the molecular mass of 36 kDa and 33 kDa respectively (PMID: 14732705, 28178523).

Protocols

Product Specific Protocols
WB protocol for PARL antibody 85167-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols