PARK7/DJ-1 Recombinant antibody

PARK7/DJ-1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

230124E12

Cat no : 82913-1-RR

Synonyms

PARK7, PARK7,DJ-1, Maillard deglycase, EC:3.1.2.-, EC:3.5.1.-, EC:3.5.1.124, DJ-1

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Validation Data Gallery

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  WB analysis using 82913-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82913-1-RR (PARK7/DJ-1 antibody) at dilution of 1:18000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 82913-1-RR

  IP result of anti-PARK7/DJ-1 (IP:82913-1-RR, 4ug; Detection:82913-1-RR 1:2000) with HeLa cells lysate 1920 ug.

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  IF Staining of HepG2 using 82913-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PARK7/DJ-1 antibody (82913-1-RR, Clone: 230124E12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HepG2 using 82913-1-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PARK7/DJ-1 Recombinant antibody (82913-1-RR, Clone:230124E12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HeLa using 82913-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug PARK7/DJ-1 Recombinant antibody (82913-1-RR, Clone:230124E12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82913-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82913-1-RR against Human PARK7/DJ-1 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82913-1-RR targets PARK7/DJ-1 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PARK7/DJ-1 fusion protein Ag2287
• Full Name: Parkinson disease (autosomal recessive, early onset) 7
• Calculated Molecular Weight: 189 aa, 20 kDa
• Observed Molecular Weight: 20-25 kDa
• GenBank Accession Number: BC008188
• Gene Symbol: PARK7
• Gene ID (NCBI): 11315
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PARK7, also named as DJ1, belongs to the peptidase C56 family. It protects cells against oxidative stress and cell death. PARK7 plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. It eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. PARK7 has cell-growth promoting activity and transforming activity. It may function as a redox-sensitive chaperone. It's precursor undergoes a cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. The amino acid replace at 166 (L → P) reduces PARK7 protein stability and leads to increased degradation. The predicted MW of this protein is 20 kDa, An additional 25 kDa band can be observed due to modification (PMID: 31767755).