PARK7/DJ-1 Recombinant antibody, PBS Only (Capture)

PARK7/DJ-1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), IP, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230124E12

Cat no : 82913-1-PBS

Synonyms

PARK7, PARK7,DJ-1, Maillard deglycase, EC:3.1.2.-, EC:3.5.1.-, EC:3.5.1.124, DJ-1

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Validation Data Gallery

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  WB analysis using 82913-1-RR (same clone as 82913-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82913-1-RR (PARK7/DJ-1 antibody) at dilution of 1:18000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82913-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP80004-1

  Sandwich ELISA standard curve of MP80004-1, Human PARK7/DJ-1 Monoclonal Matched Antibody Pair - PBS only. 82913-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2287. 68802-1-PBS was HRP conjugated as the detection antibody. Range: 0.39-25 ng/mL.

• 

  Sample test of MP80004-1

  The mean human PARK7/DJ-1 concentration was determined to be 14.25 ng/mL in HL-60 cell extract based on a 4.3 mg/mL extract load.

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  Sample test of MP80004-1

  The human PARK7/DJ-1 concentration of Human cerebrospinal fluid (CSF) samples were determined to be 8.3 ng/mL.

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  Sample test of MP80004-1

  Serum of sixteen individual healthy human donors were measured. The human PARK7/DJ-1 concentration of detected samples was determined to be 28.3 ng/mL with a range of 6.3 - 44.6 ng/mL.

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  IP experiment of HeLa using 82913-1-RR (same clone as 82913-1-PBS)

  IP result of anti-PARK7/DJ-1 (IP:82913-1-RR, 4ug; Detection:82913-1-RR 1:2000) with HeLa cells lysate 1920 ug. This data was developed using the same antibody clone with 82913-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82913-1-RR (same clone as 82913-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PARK7/DJ-1 antibody (82913-1-RR, Clone: 230124E12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82913-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 82913-1-RR (same clone as 82913-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PARK7/DJ-1 Recombinant antibody (82913-1-RR, Clone:230124E12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82913-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 82913-1-RR (same clone as 82913-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug PARK7/DJ-1 Recombinant antibody (82913-1-RR, Clone:230124E12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82913-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82913-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82913-1-RR against Human PARK7/DJ-1 were performed. The affinity constant is below 1 pM.

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Product Information

82913-1-PBS targets PARK7/DJ-1 as part of a matched antibody pair:

MP80004-1: 82913-1-PBS capture and 68802-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PARK7/DJ-1 fusion protein Ag2287
• Full Name: Parkinson disease (autosomal recessive, early onset) 7
• Calculated Molecular Weight: 189 aa, 20 kDa
• Observed Molecular Weight: 20-25 kDa
• GenBank Accession Number: BC008188
• Gene Symbol: PARK7
• Gene ID (NCBI): 11315
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PARK7, also named as DJ1, belongs to the peptidase C56 family. It protects cells against oxidative stress and cell death. PARK7 plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. It eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. PARK7 has cell-growth promoting activity and transforming activity. It may function as a redox-sensitive chaperone. It's precursor undergoes a cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. The amino acid replace at 166 (L → P) reduces PARK7 protein stability and leads to increased degradation. The predicted MW of this protein is 20 kDa, An additional 25 kDa band can be observed due to modification (PMID: 31767755).