CoraLite®594-conjugated PARK2/Parkin Polyclonal antibody

• Featured Product
• KD/KO Validated

PARK2/Parkin Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-14060

Synonyms

AR JP, LPRS2, PARK2, Parkinson disease protein 2, PDJ, PRKN

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Validation Data Gallery

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  IF Staining of HeLa using CL594-14060

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 PARK2/Parkin antibody (CL594-14060) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-14060 targets PARK2/Parkin in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PARK2/Parkin fusion protein Ag5092
• Full Name: Parkinson disease (autosomal recessive, juvenile) 2, parkin
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 42-52 kDa
• GenBank Accession Number: BC022014
• Gene Symbol: Parkin
• Gene ID (NCBI): 5071
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Parkin, a RING-type E3 ubiquitin-protein ligase, is involved in the ubiquitination pathway and contributes to protection from neurotoxicity induced by unfolded protein stresses. Its ubiquitin-protein ligase activity promotes the degradation of a variety of proteins including itself. Mutations in Parkin are implicated in the pathogenesis of autosomal recessive familial Parkinson's disease. It has 8 isoforms produced by alternative splicing.