CoraLite® Plus 488-conjugated PARD3 Polyclonal antibody

• Featured Product
• KD/KO Validated

PARD3 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11085

Synonyms

ASIP, Baz, Bazooka, CTCL tumor antigen se2 5, FLJ21015, PAR 3, PAR3, PAR3 alpha, PAR3A, PAR3alpha, PARD 3, PARD3, PARD3A, SE2 5L16, SE2 5LT1, SE2 5T2

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Validation Data Gallery

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  IF Staining of mouse colon using CL488-11085

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CoraLite® Plus 488 PARD3 antibody (CL488-11085) at dilution of 1:100.

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  IF Staining of mouse colon using CL488-11085

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CoraLite® Plus 488 PARD3 antibody (CL488-11085) at dilution of 1:100.

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Tested Applications

• Positive IF-P detected in: mouse colon tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11085 targets PARD3 in IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PARD3 fusion protein Ag1565
• Full Name: par-3 partitioning defective 3 homolog (C. elegans)
• Calculated Molecular Weight: 151 kDa
• Observed Molecular Weight: 180 kDa, 140-150 kDa, 100 kDa
• GenBank Accession Number: BC011711
• Gene Symbol: PARD3
• Gene ID (NCBI): 56288
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PARD3 (also known as ASIP, Par3, or Bazooka) is one of PARD proteins which are essential for asymmetric cell division and polarized growth. PARD3 is involved in the establishment of cell polarity and in the asymmetric cytokinesis. It plays a role in tight junctions at epithelial cell-cell contacts. PARD3 has three splice isoforms at 100 kDa, 150 kDa, and 180 kDa. This polyclonal antibody raised against C-terminal 281 amino acids of human PARD3 recognizes these three isoforms.