CoraLite® Plus 488-conjugated PAK6 Monoclonal antibody

PAK6 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3G8C1

Cat no : CL488-67562

Synonyms

p21 activated kinase 6, PAK 5, PAK 6, PAK5, PAK6

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Validation Data Gallery

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  IF Staining of HEK-293 using CL488-67562

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 PAK6 antibody (CL488-67562, Clone: 3G8C1 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67562 targets PAK6 in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PAK6 fusion protein Ag27692
• Full Name: p21 protein (Cdc42/Rac)-activated kinase 6
• Calculated Molecular Weight: 681 aa, 75 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC035596
• Gene Symbol: PAK6
• Gene ID (NCBI): 56924
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PAK6(p21-activated kinase 6) is a serine/threonine kinase belonging to the p21-activated kinase (PAK) family. .This protein plays a role in the regulation of gene transcription and may protect cells from apoptosis through phosphorylation of BAD. Northern blot analysis revealed that PAK6 is highly expressed in testis and prostate tissues (PMID:11278661).