PAK4 Recombinant antibody, PBS Only

PAK4 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240686D1

Cat no : 83761-5-PBS

Synonyms

PAK 4, p21-activated kinase 4, p21 activated kinase 4, KIAA1142, EC:2.7.11.1

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Validation Data Gallery

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  WB analysis using 83761-5-RR (same clone as 83761-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83761-5-RR (PAK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83761-5-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 83761-5-RR (same clone as 83761-5-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PAK4 Recombinant antibody (83761-5-RR, Clone:240686D1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83761-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83761-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83761-5-RR against Human PAK4 were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 83761-5-PBS is PAK4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PAK4 fusion protein Ag5981
• Full Name: p21 protein (Cdc42/Rac)-activated kinase 4
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC025282
• Gene Symbol: PAK4
• Gene ID (NCBI): 10298
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PAK4(p21-activated kinase 4) is also named as KIAA1142. It belongs to the protein kinase superfamily.PAK4 regulates cell morphology, cytoskeletal organization, and cell proliferation and migration. It can also function as an antiapoptotic protein (PMID:14517283). It has 4 isoforms produced by alternative splicing. And it can be autophosphorylated on serine residues when activated by CDC42/p21 (PMID:11278822).