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PAK4 Recombinant antibody

PAK4 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240686D1

Cat no : 83761-5-RR

Synonyms

PAK 4, p21-activated kinase 4, p21 activated kinase 4, KIAA1142, EC:2.7.11.1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PAK4 Recombinant antibody (83761-5-RR)

    WB analysis using 83761-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83761-5-RR (PAK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of MCF-7 cells using PAK4 Recombinant antibody (83761-5-RR)

    FC experiment of MCF-7 using 83761-5-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PAK4 Recombinant antibody (83761-5-RR, Clone:240686D1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83761-5-RR

    Affinity and Kinetic Characterization of 83761-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83761-5-RR against Human PAK4 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHeLa cells, COLO 320 cells, Raji cells, MCF-7 cells, PC-3 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83761-5-RR is PAK4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PAK4 fusion protein Ag5981
Full Name p21 protein (Cdc42/Rac)-activated kinase 4
Calculated Molecular Weight 64 kDa
Observed Molecular Weight64 kDa
GenBank Accession NumberBC025282
Gene Symbol PAK4
Gene ID (NCBI) 10298
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAK4(p21-activated kinase 4) is also named as KIAA1142. It belongs to the protein kinase superfamily.PAK4 regulates cell morphology, cytoskeletal organization, and cell proliferation and migration. It can also function as an antiapoptotic protein (PMID:14517283). It has 4 isoforms produced by alternative splicing. And it can be autophosphorylated on serine residues when activated by CDC42/p21 (PMID:11278822).

Protocols

Product Specific Protocols
WB protocol for PAK4 antibody 83761-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols