PADI2 Monoclonal antibody, PBS Only (Capture)

• Featured Product
• KD/KO Validated

PADI2 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D8C12

Cat no : 66386-1-PBS

Synonyms

PADI2 protein, PAD-H19, PAD2, PAD H19, KIAA0994

---

Validation Data Gallery

• 

  WB analysis of human skeletal muscle using 66386-1-Ig (same clone as 66386-1-PBS)

  human skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66386-1-Ig (PADI2 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 66386-1-Ig (same clone as 66386-1-PBS)

  WB result of PADI2 antibody (66386-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PADI2 transfected MCF-7 cells. This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66386-1-Ig (same clone as 66386-1-PBS)

  WB result of PADI2 antibody (66386-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PADI2 transfected HeLa cells. This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66386-1-Ig (same clone as 66386-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66386-1-Ig (PADI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66386-1-Ig (same clone as 66386-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66386-1-Ig (PADI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 66386-1-Ig (same clone as 66386-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66386-1-Ig (PADI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66386-1-Ig (same clone as 66386-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66386-1-Ig (PADI2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66386-1-Ig (same clone as 66386-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66386-1-Ig (PADI2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 66386-1-Ig (same clone as 66386-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66386-1-Ig (PADI2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50376-2

  Cytometric bead array standard curve of MP50376-2, PADI2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66386-1-PBS. Detection antibody: 66386-3-PBS. Standard:Ag17612. Range: 0.781-100 ng/mL.

---

Product Information

66386-1-PBS targets PADI2 as part of a matched antibody pair:

MP50376-2: 66386-1-PBS capture and 66386-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PADI2 fusion protein Ag17612
• Full Name: peptidyl arginine deiminase, type II
• Calculated Molecular Weight: 665 aa, 75 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC009701
• Gene Symbol: PADI2
• Gene ID (NCBI): 11240
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PADI2, also named as KIAA0994, PDI2, PAD-H19 and PAD2(Peptidylarginine deiminase II ), belongs to the protein arginine deiminase family. It catalyzes the deimination of arginine residues of proteins. PADI2 may play a regulatory role in the expression of lactation related genes via histone citrullination during diestrus (PMID:20668670). PADI2 has two isoforms with MW 75 kDa and 49 kDa.