PACSIN1 Monoclonal antibody, PBS Only

PACSIN1 Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Chicken, Pig

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B7B4

Cat no : 68115-1-PBS

Synonyms

KIAA1379, PACSIN1, SDPI, Syndapin-1

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Validation Data Gallery

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  WB analysis using 68115-1-Ig (same clone as 68115-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68115-1-Ig (PACSIN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68115-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 68115-1-Ig (same clone as 68115-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68115-1-Ig (PACSIN1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68115-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 68115-1-Ig (same clone as 68115-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68115-1-Ig (PACSIN1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68115-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68115-1-Ig (same clone as 68115-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68115-1-Ig (PACSIN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68115-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68115-1-Ig (same clone as 68115-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68115-1-Ig (PACSIN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68115-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 68115-1-Ig (same clone as 68115-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using PACSIN1 antibody (68115-1-Ig, Clone: 1B7B4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68115-1-PBS in a different storage buffer formulation.

• 

  FC experiment of SH-SY5Y using 68115-1-Ig (same clone as 68115-1-PBS)

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human PACSIN1 (68115-1-Ig, Clone:1B7B4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68115-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68115-1-PBS targets PACSIN1 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Chicken, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Rabbit, Chicken, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PACSIN1 fusion protein Ag4102
• Full Name: protein kinase C and casein kinase substrate in neurons 1
• Calculated Molecular Weight: 444 aa, 51 kDa
• Observed Molecular Weight: 48-51 kDa
• GenBank Accession Number: BC040228
• Gene Symbol: PACSIN1
• Gene ID (NCBI): 29993
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PACSIN1 (also known as syndapin-1) is a member of the protein kinase C and casein kinase substrate in neurons (PACSIN) family. In mammals, the PACSIN family is comprised of three members, PACSIN1, PACSIN2, and PACSIN3 (PMID: 34990060). PACSIN1 is expressed mainly in neurons, whereas PACSIN2 is ubiquitously expressed in all tissues, and PACSIN3 is expressed mainly in skeletal muscle and the heart (PMID: 23668323). All of these three members contain an N-terminal F-BAR domain and a C-terminal SH3 domain. PACSIN1 plays a role in endocytosis and endosomal recycling. Meanwhile, it has a role in actin remodeling and microtubule nucleation and also plays a particular role in membrane shaping and reconstruction (PMID: 23035120; 34422904). PACSIN1 is involved in neuromorphogenesis and the regulation of the nervous system, and the inappropriate expression of PACSIN1 has been associated with some neurological diseases, including schizophrenia, Alzheimer's disease, and Huntington's disease (PMID: 34990060).