P16-INK4A Polyclonal antibody

P16-INK4A Polyclonal Antibody for WB, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 30519-1-AP

Synonyms

CDKN2A, MTS1, CDKN2, CDK4I, CDK4 inhibitor p16INK4

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Validation Data Gallery

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  WB analysis using 30519-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30519-1-AP (P16-INK4A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 30519-1-AP

  IP result of anti-P16-INK4A (IP:30519-1-AP, 4ug; Detection:30519-1-AP 1:1000) with HEK-293 cells lysate 1280 ug.

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  FC experiment of HeLa using 30519-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.8 ug Anti-Human P16-INK4A (30519-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IP detected in: HEK-293 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

30519-1-AP targets P16-INK4A in WB, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: P16-INK4A fusion protein Ag29567
• Full Name: cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: NM_000077
• Gene Symbol: P16-INK4A
• Gene ID (NCBI): 1029
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

P16-INK4A is also named as CDKN2A, MLM, Tumor suppressor ARF, Alternative reading frame. The tumor suppressor protein p16Ink4a (encoded from the CDKN2A locus) is often transcriptionally activated in cells undergoing senescence and is one of the main regulators of this program, and it is upregulated in multiple tissues during aging (PMID:17055429). p16-Ink4a is the principal member of the Ink4 family of CDK inhibitors. p16-Ink4a contributes to the regulation of cell cycle progression by inhibiting the S phase. p16Ink4a binds to CDK4/6, inhibiting cyclin D-CDK4/6 complex formation and CDK4/6-mediated phosphorylation of Rb family members. Expression of p16-Ink4a maintains the Rb family members in a hypophosphorylated state, which promotes binding to E2F1 and leads to G1 cell cycle arrest (PMID: 21297668).

Publications

Species	Application	Title
human	WB	Biochim Biophys Acta Mol Cell Res; Suppression of GATA3 promotes epithelial-mesenchymal transition and simultaneous cellular senescence in human extravillous trophoblasts; Authors - En-Xiang Chen