CoraLite® Plus 488-conjugated OXCT1 Recombinant antibody

OXCT1 Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4K12

Cat no : CL488-81011

Synonyms

SCOT, OXCT, EC:2.8.3.5, 4K12, 3-oxoacid CoA-transferase 1

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Validation Data Gallery

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  IF Staining of HeLa using CL488-81011

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 OXCT1 antibody (CL488-81011, Clone: 4K12 ) at dilution of 1:200.

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  IF Staining of MCF-7 using CL488-81011

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 OXCT1 antibody (CL488-81011, Clone: 4K12 ) at dilution of 1:200.

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  FC experiment of MCF-7 using CL488-81011

  1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human OXCT1 (CL488-81011, Clone:4K12) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells, MCF-7 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-81011 targets OXCT1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: OXCT1 fusion protein Ag2818
• Full Name: 3-oxoacid CoA transferase 1
• Calculated Molecular Weight: 520 aa, 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC009001
• Gene Symbol: OXCT1
• Gene ID (NCBI): 5019
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

3-oxoacid-CoA transferase 1 (OXCT1), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)