HUVEC cells were subjected to SDS PAGE followed by western blot with 82794-7-RR (OX40L/TNFSF4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
HUVEC cells were subjected to SDS PAGE followed by western blot with 82794-7-RR (OX40L/TNFSF4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IF Staining of MJ using 82794-7-RR
Immunofluorescent analysis of (4% PFA) fixed MJ cells using OX40L/TNFSF4 antibody (82794-7-RR, Clone: 241813A6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (4% PFA) fixed MJ cells using OX40L/TNFSF4 antibody (82794-7-RR, Clone: 241813A6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC experiment of MJ using 82794-7-RR
1x10^6 MJ cells were surface stained with 0.25 ug OX40L/TNFSF4 Recombinant antibody (82794-7-RR, Clone:241813A6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were not fixed.
1x10^6 MJ cells were surface stained with 0.25 ug OX40L/TNFSF4 Recombinant antibody (82794-7-RR, Clone:241813A6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were not fixed.
Affinity and Kinetic Characterization of 82794-7-RR
Biolayer interferometry (BLl) kinetic assays of 82794-7-RR against Human OX40L/TNFSF4 were performed. The affinity constant is 13.8 pM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
HUVEC cells were subjected to SDS PAGE followed by western blot with 82794-7-RR (OX40L/TNFSF4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
FC Figures
FC experiment of MJ using 82794-7-RR
1x10^6 MJ cells were surface stained with 0.25 ug OX40L/TNFSF4 Recombinant antibody (82794-7-RR, Clone:241813A6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were not fixed.
IF/ICC Figures
IF Staining of MJ using 82794-7-RR
Immunofluorescent analysis of (4% PFA) fixed MJ cells using OX40L/TNFSF4 antibody (82794-7-RR, Clone: 241813A6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
AFFINITY Figures
Affinity and Kinetic Characterization of 82794-7-RR
Biolayer interferometry (BLl) kinetic assays of 82794-7-RR against Human OX40L/TNFSF4 were performed. The affinity constant is 13.8 pM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
OX40L/TNFSF4 Recombinant antibody
Catalog Number
82794-7-RR
Citations
-
Dilutions
WB : 1:1000-1:8000 IF/ICC : 1:125-1:500
Applications
WB, IF/ICC, ELISA
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
