OTUB1 Monoclonal antibody, PBS Only

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  WB analysis using 68489-1-Ig (same clone as 68489-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68489-1-Ig (OTUB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68489-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 68489-1-Ig (same clone as 68489-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using OTUB1 antibody (68489-1-Ig, Clone: 2F7F8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68489-1-PBS in a different storage buffer formulation.

OTUB1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 68489-1-PBS

Clone No.2F7F8

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

OTU domain-containing ubiquitin aldehyde-binding protein 1, OTB1, HSPC263, hOTU1, EC:3.4.19.12

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Product Information

68489-1-PBS targets OTUB1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: OTUB1 fusion protein Ag33132
• Full Name: OTU domain, ubiquitin aldehyde binding 1
• Calculated Molecular Weight: 31 kDa and 35 kDa
• Observed Molecular Weight: 31-35 kDa
• GenBank Accession Number: BC007519
• Gene Symbol: OTUB1
• Gene ID (NCBI): 55611
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96FW1
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

OTUB1 belongs to the ovarian tumor domain protease (OTU) subfamily of deubiquitinases, which could block ubiquitination leading to protein stability. OTUB1 mainly prefers k-48 and k-63 ubiquitin chains type, especially in k-48 ubiquitin chain. OTUB1 performs its deubiquitinating function via two distinct manners: the canonical and non-canonical manner. OTUB1 is involved in many critical immune response processes, containing CD8 T cell activation, PD-L1 related immune evasion, and several infectious diseases. OTUB1 plays an important role in the occurrence, proliferation, invasion, and progression of various cancers, such as bladder cancer, breast cancer, prostate cancer, and colorectal cancer (PMID: 34875341, 35296795). OTUB1 has 2 isoforms with molecular weights of 31 and 35 kDa, respectively.