OTUB1 Monoclonal antibody

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  WB analysis using 68489-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68489-1-Ig (OTUB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  IF Staining of U2OS using 68489-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using OTUB1 antibody (68489-1-Ig, Clone: 2F7F8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

OTUB1 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 68489-1-Ig

Clone No.2F7F8

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

OTU domain-containing ubiquitin aldehyde-binding protein 1, OTB1, HSPC263, hOTU1, EC:3.4.19.12

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Tested Applications

• Positive WB detected in: U2OS cells, LNCaP cells, HeLa cells, HEK-293 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68489-1-Ig targets OTUB1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: OTUB1 fusion protein Ag33132
• Full Name: OTU domain, ubiquitin aldehyde binding 1
• Calculated Molecular Weight: 31 kDa and 35 kDa
• Observed Molecular Weight: 31-35 kDa
• GenBank Accession Number: BC007519
• Gene Symbol: OTUB1
• Gene ID (NCBI): 55611
• RRID: AB_3085200
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96FW1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OTUB1 belongs to the ovarian tumor domain protease (OTU) subfamily of deubiquitinases, which could block ubiquitination leading to protein stability. OTUB1 mainly prefers k-48 and k-63 ubiquitin chains type, especially in k-48 ubiquitin chain. OTUB1 performs its deubiquitinating function via two distinct manners: the canonical and non-canonical manner. OTUB1 is involved in many critical immune response processes, containing CD8 T cell activation, PD-L1 related immune evasion, and several infectious diseases. OTUB1 plays an important role in the occurrence, proliferation, invasion, and progression of various cancers, such as bladder cancer, breast cancer, prostate cancer, and colorectal cancer (PMID: 34875341, 35296795). OTUB1 has 2 isoforms with molecular weights of 31 and 35 kDa, respectively.