OTC Monoclonal antibody, PBS Only

OTC Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F11C7

Cat no : 67553-1-PBS

Synonyms

OCTD, ornithine carbamoyltransferase, Ornithine transcarbamylase, OTC, OTCase

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Validation Data Gallery

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  WB analysis using 67553-1-Ig (same clone as 67553-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67553-1-Ig (OTC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67553-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67553-1-Ig (same clone as 67553-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67553-1-Ig (OTC antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67553-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67553-1-Ig (same clone as 67553-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67553-1-Ig (OTC antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67553-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 67553-1-Ig (same clone as 67553-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67553-1-Ig (OTC antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67553-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67553-1-PBS targets OTC in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: OTC fusion protein Ag24186
• Full Name: ornithine carbamoyltransferase
• Calculated Molecular Weight: 354 aa, 40 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC074745
• Gene Symbol: OTC
• Gene ID (NCBI): 5009
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Ornithine transcarbamylase (OTC) is a liver and intestinal mucosa-located, intramitochondrial, rate-limiting enzyme in the urea cycle. It catalyzes the reaction that converts ornithine and carbamoyl phosphate into citrulline, which is the second step of the urea cycle, to detoxify the ammonia produced from amino acid catabolism.