OTC Monoclonal antibody

OTC Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

2F11C7

Cat no : 67553-1-Ig

Synonyms

OCTD, ornithine carbamoyltransferase, Ornithine transcarbamylase, OTC, OTCase

---

Validation Data Gallery

• 

  WB analysis using 67553-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67553-1-Ig (OTC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 67553-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67553-1-Ig (OTC antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67553-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67553-1-Ig (OTC antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 67553-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67553-1-Ig (OTC antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: pig liver tissue, rat liver tissue, mouse liver tissue
• Positive IHC detected in: human liver cancer tissue, human liver tissue, rat colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:4000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

67553-1-Ig targets OTC in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: OTC fusion protein Ag24186
• Full Name: ornithine carbamoyltransferase
• Calculated Molecular Weight: 354 aa, 40 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC074745
• Gene Symbol: OTC
• Gene ID (NCBI): 5009
• RRID: AB_2882767
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ornithine transcarbamylase (OTC) is a liver and intestinal mucosa-located, intramitochondrial, rate-limiting enzyme in the urea cycle. It catalyzes the reaction that converts ornithine and carbamoyl phosphate into citrulline, which is the second step of the urea cycle, to detoxify the ammonia produced from amino acid catabolism.

Publications

Species	Application	Title
mouse	WB	Sci Transl Med; Aberrant methylmalonylation underlies methylmalonic acidemia and is attenuated by an engineered sirtuin; Authors - PamelaSara E Head
human	WB	Biomater Res; Genetically Modified Hepatocytes Targeting Bilirubin and Ammonia Metabolism for the Construction of Bioartificial Liver System; Authors - Ke Wang