ORM2-Specific Monoclonal antibody, PBS Only

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  WB analysis of human plasma using 66217-1-Ig (same clone as 66217-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66217-1-Ig (ORM2-Specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66217-1-PBS in a different storage buffer formulation.

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  WB analysis of Recombinant protein using 66217-1-Ig (same clone as 66217-1-PBS)

  Recombinant protein of ORM1 and ORM2 were subjected to SDS PAGE followed by western blot with 66217-1-Ig (ORM2-Specific antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66217-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 66217-1-Ig (same clone as 66217-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66217-1-Ig (ORM2-Specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66217-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66217-1-Ig (same clone as 66217-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66217-1-Ig (ORM2-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66217-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66217-1-Ig (same clone as 66217-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66217-1-Ig (ORM2-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66217-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66217-1-Ig (same clone as 66217-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66217-1-Ig (ORM2-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66217-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66217-1-Ig (same clone as 66217-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66217-1-Ig (ORM2-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66217-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66217-1-Ig (same clone as 66217-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ORM2-Specific antibody (66217-1-Ig, Clone: 4E9H10 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66217-1-PBS in a different storage buffer formulation.

ORM2-Specific Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66217-1-PBS

Clone No.4E9H10

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ORM1/2, ORM2, AGPB, AGP2, AGP B'

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Product Information

66217-1-PBS targets ORM2-Specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ORM2-Specific fusion protein Ag18984
• Full Name: orosomucoid 2
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC015964
• Gene Symbol: ORM2
• Gene ID (NCBI): 5005
• RRID: AB_2881608
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P19652
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

ORM2, also named as AGP2 and OMD 2, belongs to the calycin superfamily and Lipocalin family. It functions as transport protein in the blood stream. ORM2 binds various hydrophobic ligands in the interior of its beta-barrel domain. It also binds synthetic drugs and influences their distribution and availability. ORM2 appears to function in modulating the activity of the immune system during the acute-phase reaction. ORM2 is one of the conserved endoplasmic reticulum membrane proteins which regulating lipid homeostasis and protein quality control. This antibody can specially recognize ORM2. It recognizes a band about 45 kDa in human plasma which may be due to the glycosylation of ORM2 or the dimer formation of the protein.