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ORM2-Specific Monoclonal antibody

ORM2-Specific Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4E9H10

Cat no : 66217-1-Ig

Synonyms

AGP B', AGP2, Alpha 1 acid glycoprotein 2, ORM2, ORM2-Specific

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using ORM2-Specific Monoclonal antibody (66217-1-Ig)

    WB analysis of human plasma using 66217-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66217-1-Ig (ORM2-Specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Recombinant protein using ORM2-Specific Monoclonal antibody (66217-1-Ig)

    WB analysis of Recombinant protein using 66217-1-Ig

    Recombinant protein of ORM1 and ORM2 were subjected to SDS PAGE followed by western blot with 66217-1-Ig (ORM2-Specific antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using ORM2-Specific Monoclonal antibody (66217-1-Ig)

    WB analysis of A549 using 66217-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 66217-1-Ig (ORM2-Specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ORM2-Specific Monoclonal antibody (66217-1-Ig)

    IHC staining of human liver cancer using 66217-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66217-1-Ig (ORM2-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ORM2-Specific Monoclonal antibody (66217-1-Ig)

    IHC staining of human liver cancer using 66217-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66217-1-Ig (ORM2-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ORM2-Specific Monoclonal antibody (66217-1-Ig)

    IHC staining of human colon cancer using 66217-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66217-1-Ig (ORM2-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ORM2-Specific Monoclonal antibody (66217-1-Ig)

    IHC staining of human colon cancer using 66217-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66217-1-Ig (ORM2-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ORM2-Specific Monoclonal antibody (66217-1-Ig)

    IF Staining of HepG2 using 66217-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66217-1-Ig(ORM2-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman plasma tissue, A549 cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66217-1-Ig targets ORM2-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ORM2-Specific fusion protein Ag18984
Full Name orosomucoid 2
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC015964
Gene Symbol ORM2
Gene ID (NCBI) 5005
RRIDAB_2881608
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ORM2, also named as AGP2 and OMD 2, belongs to the calycin superfamily and Lipocalin family. It functions as transport protein in the blood stream. ORM2 binds various hydrophobic ligands in the interior of its beta-barrel domain. It also binds synthetic drugs and influences their distribution and availability. ORM2 appears to function in modulating the activity of the immune system during the acute-phase reaction. ORM2 is one of the conserved endoplasmic reticulum membrane proteins which regulating lipid homeostasis and protein quality control. This antibody can specially recognize ORM2. It recognizes a band about 45 kDa in human plasma which may be due to the glycosylation of ORM2 or the dimer formation of the protein.

Protocols

Product Specific Protocols
WB protocol for ORM2-Specific antibody 66217-1-IgDownload protocol
IHC protocol for ORM2-Specific antibody 66217-1-IgDownload protocol
IF protocol for ORM2-Specific antibody 66217-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols