ORM1/2 Polyclonal antibody, PBS Only

ORM1/2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11199-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 11199-1-AP (same clone as 11199-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 11199-1-AP (ORM2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11199-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 11199-1-AP (same clone as 11199-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11199-1-AP (ORM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11199-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 11199-1-AP (same clone as 11199-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 11199-1-AP (ORM2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11199-1-PBS in a different storage buffer formulation.

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  IP experiment of L02 using 11199-1-AP (same clone as 11199-1-PBS)

  IP result of anti-ORM1/2 (IP:11199-1-AP, 3ug; Detection:11199-1-AP 1:800) with L02 cells lysate 2500ug. This data was developed using the same antibody clone with 11199-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 11199-1-AP (same clone as 11199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11199-1-AP (ORM1/2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11199-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 11199-1-AP (same clone as 11199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11199-1-AP (ORM1/2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11199-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 11199-1-AP (same clone as 11199-1-PBS)

  Immunofluorescent analysis of Hela cells, using ORM2 antibody 11199-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 11199-1-PBS in a different storage buffer formulation.

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Product Information

11199-1-PBS targets ORM1/2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ORM1/2 fusion protein Ag1667
• Full Name: orosomucoid 2
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 24-28 kDa, 41-47 kDa
• GenBank Accession Number: BC015964
• Gene Symbol: ORM2
• Gene ID (NCBI): 5005
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ORM2, also named as AGP2 and OMD 2, belongs to the calycin superfamily and Lipocalin family. It functions as transport protein in the blood stream. ORM2 binds various hydrophobic ligands in the interior of its beta-barrel domain. It also binds synthetic drugs and influences their distribution and availability. ORM2 appears to function in modulating the activity of the immune system during the acute-phase reaction. ORM2 is one of the conserved endoplasmic reticulum membrane proteins which regulating lipid homeostasis and protein quality control. This antibody can recognize both ORM1 and ORM2.