ORM1/2 Polyclonal antibody

ORM1/2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 11199-1-AP

Synonyms

ORM2, AGPB', AGPB, AGP2, AGP B'

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Validation Data Gallery

• 

  WB analysis using 11199-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11199-1-AP (ORM2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11199-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11199-1-AP (ORM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11199-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11199-1-AP (ORM2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 11199-1-AP

  IP result of anti-ORM1/2 (IP:11199-1-AP, 3ug; Detection:11199-1-AP 1:800) with L02 cells lysate 2500ug.

• 

  IHC staining of human liver using 11199-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11199-1-AP (ORM1/2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 11199-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11199-1-AP (ORM1/2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11199-1-AP

  Immunofluorescent analysis of Hela cells, using ORM2 antibody 11199-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: L02 cells, A549 cells, mouse liver tissue
• Positive IP detected in: L02 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• ChIP: See 1 publications below

Product Information

11199-1-AP targets ORM1/2 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ORM1/2 fusion protein Ag1667
• Full Name: orosomucoid 2
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 24-28 kDa, 41-47 kDa
• GenBank Accession Number: BC015964
• Gene Symbol: ORM2
• Gene ID (NCBI): 5005
• RRID: AB_2158180
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ORM2, also named as AGP2 and OMD 2, belongs to the calycin superfamily and Lipocalin family. It functions as transport protein in the blood stream. ORM2 binds various hydrophobic ligands in the interior of its beta-barrel domain. It also binds synthetic drugs and influences their distribution and availability. ORM2 appears to function in modulating the activity of the immune system during the acute-phase reaction. ORM2 is one of the conserved endoplasmic reticulum membrane proteins which regulating lipid homeostasis and protein quality control. This antibody can recognize both ORM1 and ORM2.

Publications

Species	Application	Title
mouse		Carcinogenesis; FXR-Gankyrin axis is involved in development of pediatric liver cancer.; Authors - Leila Valanejad
mouse	WB	Mol Cell Biol; RNA Binding Protein CUGBP1 Inhibits Liver Cancer in a Phosphorylation-Dependent Manner.; Authors - Kyle Lewis
human	WB	BMC Cancer; Differential profiling of breast cancer plasma proteome by isotope-coded affinity tagging method reveals biotinidase as a breast cancer biomarker.; Authors - Kang Un-Beom UB
mouse	IHC	Sci Rep; Periodic heat waves-induced neuronal etiology in the elderly is mediated by gut-liver-brain axis: a transcriptome profiling approach; Authors - Subhajit Roy
mouse	ChIP	Am J Pathol; TRIM24 Up-Regulates ORM2 to Alleviate Abnormal Lipid Metabolism, Inflammation, and Oxidative Stress in Mice with Obstructive Sleep Apnea Syndrome and Metabolic Dysfunction-Associated Steatotic Liver Disease; Authors - Hui Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hanyang (Verified Customer) (03-03-2022)

Considering results we got, the capacity of antibody is regarded as suspicious.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:400

ORM1/2 Antibody Immunohistochemistry,Immunofluorescence validation (1:400 dilution) in (Cat no:11199-1-AP)

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