ORAI3 Recombinant antibody

ORAI3 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241568A2

Cat no : 84340-2-RR

Synonyms

Transmembrane protein 142C, TMEM142C, Protein orai-3, 241568A2

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Validation Data Gallery

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  WB analysis using 84340-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84340-2-RR (ORAI3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 84340-2-RR

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 84340-2-RR (ORAI3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of Jurkat using 84340-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ORAI3 antibody (84340-2-RR, Clone: 241568A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 84340-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84340-2-RR against Human ORAI3 were performed. The affinity constant is 1.24 nM.

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Tested Applications

• Positive WB detected in: Jurkat cells, MCF-7 cells, A549 cells
• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84340-2-RR is ORAI3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ORAI3 fusion protein Ag36135
• Full Name: ORAI calcium release-activated calcium modulator 3
• Calculated Molecular Weight: 295 aa, 32 kDa
• Observed Molecular Weight: ~30 kDa，~60 kDa
• GenBank Accession Number: BC006126
• Gene Symbol: ORAI3
• Gene ID (NCBI): 93129
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ORAI3, also named TMEM142C, is a key regulator or component of store-operated Ca2+ channels and transcription factor NFAT nuclear import. ORAI3 channels appeared to differ from Orai1 and -2 in being somewhat resistant to Ca2+ depotentiation. The molecular weight of monomers and dimers for Orai3 is about 30-35 kDa and 60-70 kDa (PMID: 20971921; 17293345; 22580508).