ORAI3 Recombinant antibody, PBS Only (Detector)

ORAI3 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241568A2

Cat no : 84340-2-PBS

Synonyms

Transmembrane protein 142C, TMEM142C, Protein orai-3, 241568A2

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Validation Data Gallery

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  WB analysis using 84340-2-RR (same clone as 84340-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84340-2-RR (ORAI3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84340-2-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 84340-2-RR (same clone as 84340-2-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 84340-2-RR (ORAI3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84340-2-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 84340-2-RR (same clone as 84340-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ORAI3 antibody (84340-2-RR, Clone: 241568A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84340-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01228-1

  Cytometric bead array standard curve of MP01228-1, ORAI3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84340-3-PBS. Detection antibody: 84340-2-PBS. Standard: Ag36135. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84340-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84340-2-RR against Human ORAI3 were performed. The affinity constant is 1.24 nM.

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Product Information

84340-2-PBS targets ORAI3 as part of a matched antibody pair:

MP01228-1: 84340-3-PBS capture and 84340-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ORAI3 fusion protein Ag36135
• Full Name: ORAI calcium release-activated calcium modulator 3
• Calculated Molecular Weight: 295 aa, 32 kDa
• Observed Molecular Weight: ~30 kDa，~60 kDa
• GenBank Accession Number: BC006126
• Gene Symbol: ORAI3
• Gene ID (NCBI): 93129
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ORAI3, also named TMEM142C, is a key regulator or component of store-operated Ca2+ channels and transcription factor NFAT nuclear import. ORAI3 channels appeared to differ from Orai1 and -2 in being somewhat resistant to Ca2+ depotentiation. The molecular weight of monomers and dimers for Orai3 is about 30-35 kDa and 60-70 kDa (PMID: 20971921; 17293345; 22580508).