ORAI1 Monoclonal antibody, PBS Only

ORAI1 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C12F9

Cat no : 66223-1-PBS

Synonyms

ORAI1

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Validation Data Gallery

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  WB analysis using 66223-1-Ig (same clone as 66223-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66223-1-Ig (ORAI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66223-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66223-1-Ig (same clone as 66223-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66223-1-Ig (ORAI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66223-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A375 using 66223-1-Ig (same clone as 66223-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 66223-1-Ig (ORAI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66223-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 66223-1-Ig (same clone as 66223-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ORAI1 antibody (66223-1-Ig, Clone: 3C12F9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66223-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66223-1-PBS targets ORAI1 in WB, IF, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ORAI1 fusion protein Ag18050
• Full Name: ORAI calcium release-activated calcium modulator 1
• Calculated Molecular Weight: 301 aa, 33 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC015369
• Gene Symbol: ORAI1
• Gene ID (NCBI): 84876
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a 32-55 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.