OPTN Recombinant antibody

OPTN Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240636A7

Cat no : 83634-5-RR

Synonyms

Optineurin, FIP2, FIP 2, FAA4, E3-14.7K-interacting protein

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Validation Data Gallery

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  WB analysis using 83634-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83634-5-RR (OPTN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 83634-5-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 83634-5-RR (OPTN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 83634-5-RR

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 83634-5-RR (OPTN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 83634-5-RR

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 83634-5-RR (OPTN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 83634-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug OPTN Recombinant antibody (83634-5-RR, Clone:240636A7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83634-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83634-5-RR against Human OPTN were performed. The affinity constant is 12.2 nM.

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, human testis tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83634-5-RR is OPTN Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: OPTN fusion protein Ag1272
• Full Name: optineurin
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: BC013876
• Gene Symbol: OPTN
• Gene ID (NCBI): 10133
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OPTN, also named as FIP2, GLC1E, HIP7, HYPL, and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN cause primary open angle glaucoma type 1E (GLC1E). Defects in OPTN cause susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork.