OPTN Recombinant antibody, PBS Only

OPTN Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240636A7

Cat no : 83634-5-PBS

Synonyms

Optineurin, FIP2, FIP 2, FAA4, E3-14.7K-interacting protein

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Validation Data Gallery

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  WB analysis using 83634-5-RR (same clone as 83634-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83634-5-RR (OPTN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83634-5-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 83634-5-RR (same clone as 83634-5-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 83634-5-RR (OPTN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83634-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 83634-5-RR (same clone as 83634-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 83634-5-RR (OPTN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83634-5-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 83634-5-RR (same clone as 83634-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 83634-5-RR (OPTN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83634-5-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83634-5-RR (same clone as 83634-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug OPTN Recombinant antibody (83634-5-RR, Clone:240636A7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83634-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83634-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83634-5-RR against Human OPTN were performed. The affinity constant is 12.2 nM.

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Product Information

The immunogen of 83634-5-PBS is OPTN Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: OPTN fusion protein Ag1272
• Full Name: optineurin
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: BC013876
• Gene Symbol: OPTN
• Gene ID (NCBI): 10133
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

OPTN, also named as FIP2, GLC1E, HIP7, HYPL, and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN cause primary open angle glaucoma type 1E (GLC1E). Defects in OPTN cause susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork.