OPTN/Optineurin Recombinant antibody, PBS Only

OPTN/Optineurin Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240636A7

Cat no : 83634-5-PBS

Synonyms

Optineurin, OPTN, FIP2, FIP 2, FAA4

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Validation Data Gallery

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  WB analysis using 83634-5-RR (same clone as 83634-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83634-5-RR (OPTN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83634-5-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 83634-5-RR (same clone as 83634-5-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 83634-5-RR (OPTN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83634-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 83634-5-RR (same clone as 83634-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 83634-5-RR (OPTN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83634-5-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 83634-5-RR (same clone as 83634-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 83634-5-RR (OPTN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83634-5-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83634-5-RR (same clone as 83634-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug OPTN Recombinant antibody (83634-5-RR, Clone:240636A7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83634-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83634-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83634-5-RR against Human OPTN were performed. The affinity constant is 12.2 nM.

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Product Information

83634-5-PBS targets OPTN/Optineurin in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: OPTN/Optineurin fusion protein Ag1272
• Full Name: optineurin
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: BC013876
• Gene Symbol: OPTN
• Gene ID (NCBI): 10133
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

OPTN, also named as FIP2, GLC1E, HIP7, HYPL, and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN cause susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork.