OMA1 Monoclonal antibody, PBS Only (Detector)

OMA1 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H4D12

Cat no : 67449-1-PBS

Synonyms

Overlapping with the m-AAA protease 1 homolog, MPRP-1, MPRP1, Metalloprotease-related protein 1, EC:3.4.24.-

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Validation Data Gallery

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  WB analysis using 67449-1-Ig (same clone as 67449-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67449-1-Ig (OMA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67449-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67449-1-Ig (same clone as 67449-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67449-1-Ig (OMA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67449-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67449-1-Ig (same clone as 67449-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67449-1-Ig (OMA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67449-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67449-1-Ig (same clone as 67449-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67449-1-Ig (OMA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67449-1-PBS in a different storage buffer formulation.

• 

  WB analysis of LNCaP using 67449-1-Ig (same clone as 67449-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67449-1-Ig (OMA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67449-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50571-3

  Cytometric bead array standard curve of MP50571-3, OMA1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66572-1-PBS. Detection antibody: 67449-1-PBS. Standard:Ag11201. Range: 3.125-100 ng/mL.

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Product Information

67449-1-PBS targets OMA1 as part of a matched antibody pair:

MP50571-3: 66572-1-PBS capture and 67449-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: OMA1 fusion protein Ag11201
• Full Name: OMA1 homolog, zinc metallopeptidase (S. cerevisiae)
• Calculated Molecular Weight: 524 aa, 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC012915
• Gene Symbol: OMA1
• Gene ID (NCBI): 115209
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

OMA1(Overlapping with the m-AAA protease 1 homolog) is also named as MPRP1(Metalloprotease-related protein 1) and belongs to the peptidase M48 family.It is a zinc metalloprotease that spans the inner mitochondrial membrane with a number of predicted membrane spanning domains and the 60 kDa form of OMA1 rapidly accumulated concomitantly with the cleavage of all OPA1 variants,and the 40 kDa form of OMA1 may be the inactive form, with the 60 kDa form being the active enzyme(PMID:20334834)